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bookbag

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--- name: Recitation 1 route: /2018/fall/bi341/week1/recitation/1 menu: BI 341 order: 3 --- # Chapter 1 Question: When the base composition of a DNA sample from Micrococcus luteus was determined, 37.5% of the bases were found to be cytosine. The DNA of this organism is known to be double-stranded. What is the percentage of adenine in this DNA? Question: 1.3 DNA extracted from a certain virus has the following base composition: 20 percent adenine, 40 percent thymine, 25 percent guanine, and 15 percent cytosine. How would you interpret this result in terms of the structure of the viral DNA? - The The virus has single stranded DNA. Question 1.4 Duplex DNA => *double stranded DNA*, molecule contains 642 occurences of the dinucleotide 5'-GT-3' in one or the other of the paired strands. What other dinucleotide is also present exactly 642 times? 3'-CA-5' Question 1.5 Shown here is the terminal part of a metabolic pathway in a bacterium in which a substrate metabolite (small molecule) W is converted into a final prodcut metabolite Z through a sequence of three steps catalyzed by the enzymes: A, B, and C. Each of the enzymes is the product of a differnet ... [changeed slide] Question 1.6 Mutation Question 1.7 The D.N.A. content of an organism GC base pairs On average, how frequently would you expect to find the sequence `5'ATGG3'`? ## Chapter 6 Question 6.1 The haploid genome of the wall cress, *Arabidopsis thaliana*, contains 100 Mb and has 10 chromosomes. Estimate the tiem of replication, assuming that there is only one origin of replication (exactly in the middle) for each chromosome, that replication is bidirectional, and that the rate of DNA synthesis is 1000 nucleotide pairs per second (typical of bacterial cells). 100,000,000 10 chromosomes 6.2 Specify the function or enzymatic activity of the following enzymes or enzyme complexes that participate in DNA replication: primosome, gyrase, DNA ligase, polymerase I (Pol I), and polymerase III (Pol III). | **Enzyme** | **Function** | | ---------------- | ----------------| | Primosome | DEFINITION | | Gyrase (Topoisomerase) | DEFINITION | | DNA Ligase | DEFINITION | | Polymerase I | DEFINITION | | Polymerase III | DEFINITION | 6.5 3,000,000,000 / 50,000 = **60,000**