@gmod/bed
Version:
A BED file format parser with autoSql support
161 lines (117 loc) • 4.63 kB
Markdown
# bed-js
[](https://codecov.io/gh/GMOD/bed-js/branch/main)

Performs parsing of BED files including autoSql
## Usage
### Example
You can pipe your file through this program's parseLine function
```js
import BED from '@gmod/bed'
const parser = new BED()
const text = fs.readFileSync('file.txt', 'utf8')
const results = text.split('\n').map(line => parser.parseLine(line))
```
## API
### Constructor
The BED constructor accepts an opts object with options
- opts.autoSql - a optional autoSql schema for parsing lines
- opts.type - a string representing one of a list of predefined types
The predefined types can include
bigInteract
bigMaf
bigPsl
bigNarrowPeak
bigGenePred
bigLink
bigChain
mafFrames
mafSummary
If neither autoSql or type is specified, the default BED schema is used (see
[here](src/as/autoSqlSchemas.ts))
### parseLine(line, opts)
Parses a BED line according to the currently loaded schema
- line: `string|Array<string>` - is a tab delimited line with fields from the
schema, or an array that has been pre-split by tab with those same contents
- opts: Options - an options object
An Options object can contain
- opts.uniqueId - an indication of a uniqueId that is not encoded by the BED
line itself
The default instantiation of the parser with new BED() simply parses lines
assuming the fields come from the standard BED schema. Your line can just
contain just a subset of the fields e.g.
`chrom, chromStart, chromEnd, name, score`
## Examples
### Parsing BED with default schema
```js
const p = new BED()
p.parseLine('chr1\t0\t100')
// outputs { chrom: 'chr1', chromStart: 0, chromEnd: 100, strand: 0 }
```
### Parsing BED with a built in schema e.g. bigGenePred
If you have a BED format that corresponds to a different schema, you can specify
from the list of default built in schemas
Specify this in the opts.type for the BED constructor
```js
const p = new BED({ type: 'bigGenePred' })
const line = 'chr1\t11868\t14409\tENST00000456328.2\t1000\t+\t11868\t11868\t255,128,0\t3\t359,109,1189,\t0,744,1352,\tDDX11L1\tnone\tnone\t-1,-1,-1,\tnone\tENST00000456328.2\tDDX11L1\tnone'
p.parseLine(line)
// above line outputs
{ chrom: 'chr1',
chromStart: 11868,
chromEnd: 14409,
name: 'ENST00000456328.2',
score: 1000,
strand: 1,
thickStart: 11868,
thickEnd: 11868,
reserved: '255,128,0',
blockCount: 3,
blockSizes: [ 359, 109, 1189 ],
chromStarts: [ 0, 744, 1352 ],
name2: 'DDX11L1',
cdsStartStat: 'none',
cdsEndStat: 'none',
exonFrames: [ -1, -1, -1 ],
type: 'none',
geneName: 'ENST00000456328.2',
geneName2: 'DDX11L1',
geneType: 'none' }
```
### Parsing BED with a supplied autoSql
If you have a BED format with a custom alternative schema with autoSql, or if
you are using a BigBed file that contains autoSql (e.g. with
[@gmod/bbi](https://github.com/gmod/bbi-js) then you can get it from
header.autoSql) then you initialize the schema in the constructor and then use
parseLine as normal
```
import { BigBed } from '@gmod/bbi'
const bigbed = new BigBed({path: 'yourfile'})
const {autoSql} = await bigbed.getHeader()
const p = new BED({ autoSql })
p.parseLine(line)
// etc.
```
### Important notes
- Does not parse "browser" or "track" lines and will throw an error if parseLine
receives one of these
- By default, parseLine parses only tab delimited text, if you want to use
spaces as is allowed by UCSC then pass an array to `line` for parseLine
- Converts strand from {+,-,.} to {1,-1,0} and also sets strand 0 even if no
strand is in the autoSql
## Academic Use
This package was written with funding from the [NHGRI](http://genome.gov) as
part of the [JBrowse](http://jbrowse.org) project. If you use it in an academic
project that you publish, please cite the most recent JBrowse paper, which will
be linked from [jbrowse.org](http://jbrowse.org).
## License
MIT © [Colin Diesh](https://github.com/cmdcolin)
based on
https://genome-source.gi.ucsc.edu/gitlist/kent.git/blob/master/src/hg/autoSql/autoSql.doc
also see http://genomewiki.ucsc.edu/index.php/AutoSql and
https://www.linuxjournal.com/article/5949
## Publishing
[Trusted publishing](https://docs.npmjs.com/about-trusted-publishing) via GitHub
Actions.
```bash
pnpm version patch # or minor/major
```